It is known that humans can modify the impedance of the musculoskeletal periphery but the extent of this modification is uncertain. Previous studies on impedance control under static conditions indicate a limited ability to modify impedance, whereas studies of impedance control during reaching in unstable environments suggest a greater range of impedance modification. As a first step in accounting for this difference, we have quantified the extent to which stiffness changes from posture to movement even when there are no destabilizing forces. Hand stiffness was estimated under static conditions and at the same position during both longitudinal and lateral movements using a position-servo technique. A new method was developed to predict the hand reference trajectory for purposes of estimating stiffness. For movements in a longitudinal direction, there was considerable counter-clockwise rotation of the hand stiffness ellipse relative to stiffness under static conditions. In contrast, a small counter-clockwise rotation was observed during lateral movement. In the modeling studies, even when we used the same modeled cocontraction level during posture and movement, we found that there was a substantial difference in the orientation of the stiffness ellipse, comparable to that observed empirically. Indeed, the main determinant of the orientation of the ellipse in our modeling studies was the movement direction and the muscle activation associated with movement. Changes in the cocontraction level and the balance of cocontraction had smaller effects. Thus even when there is no environmental instability, the orientation of stiffness ellipse changes during movement in a manner that varies with movement direction.