JN Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Neurophysiol 76: 554-562, 1996;
0022-3077/96 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (27)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miller, L. D.
Right arrow Articles by Connor, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, L. D.
Right arrow Articles by Connor, J. A.

Journal of Neurophysiology, Vol 76, Issue 1 554-562, Copyright © 1996 by APS

ARTICLES

Ca2+ release from intracellular stores induced by afferent stimulation of CA3 pyramidal neurons in hippocampal slices

L. D. Miller, J. J. Petrozzino, G. Golarai and J. A. Connor
Roche Institute of Molecular Biology, Roche Research Center, Nutley, New Jersey 07110-1199, USA.

1. Ca2+ imaging and simultaneous intracellular recording were performed on CA3 pyramidal neurons in hippocampal slice cultures and standard acute slices. Both fura-2 and a dextran conjugate of fura-2 (MW = 10,000) were used in the Ca2+ measurements to control for compartmentalization artifacts. Experiments were performed under conditions giving minimal ligand- and voltagegated Ca2+ influx, with the use of competitive and noncompetitive antagonists of ionotropic glutamate receptors and steady-state depolarization, respectively. 2. Tetanic stimulation of stratum lucidum evoked dendritic Ca2+ transients with rapid onset that were blocked by the noncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist, MK-801 (2-5 microM), but not by the competitive alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor antagonist, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) (10-50 microM). Zn(2+)-containing mossy fiber terminals (assessed by Timm's staining) and postsynaptic structures (thorny excrescences) are preserved in s. lucidum of hippocampal slice cultures. 3. A Ca2+ store loading protocol, consisting of brief repolarizations followed by steady depolarization, primed most of the neurons so that a subsequent tetanus gave a Ca2+ increase in the presence of MK-801 that was reported by both fura-2 and the dextran conjugate. The onset of the Ca2+ increase was significantly delayed (by 2-3 s) with respect to the MK-801-sensitive increase, and often had a different spatial pattern within the neuron. Response characteristics were similar in slice cultures and acute slices. 4. The delayed Ca2+ increase showed a steep rundown with subsequent stimuli, but was restored by further priming by the Ca2+ store loading paradigm. Postsynaptic currents evoked by the tetani under these conditions were not correlated with the magnitude of the delayed Ca2+ transients. 5. Delayed Ca2+ increases were observed in 44% of the neurons dialyzed with normal intracellular solution at room temperature. The success rate of observing delayed Ca2+ transients was increased to 86% in neurons maintained at 30 degrees C, and dialyzed with an inhibitor of the inositol-triphosphate-3-kinase. 6. The delayed Ca2+ transients could not be initiated after inhibition of endosomal Ca(2+)-ATPase-mediated uptake by thapsigargin. 7. Both fura-2 and the dextran conjugate reported increases in resting Ca2+ levels after the loading protocols, that were absent after priming in thapsigargin, and decreases in resting Ca2+ levels after successive tetani in MK-801, suggesting that the Ca2+ changes were largely cytosolic. 8. The present results support the hypothesis that these synaptically mediated, delayed Ca2+ transients represent release from intracellular Ca2+ stores that can be loaded and depleted repeatedly, and are evoked by presynaptic release of endogenous neurotransmitter.


This article has been cited by other articles:


Home page
 J. Physiol.Home page
J. S. Fitzpatrick, A. M. Hagenston, D. N. Hertle, K. E. Gipson, L. Bertetto-D'Angelo, and M. F. Yeckel
Inositol-1,4,5-trisphosphate receptor-mediated Ca2+ waves in pyramidal neuron dendrites propagate through hot spots and cold spots
J. Physiol., April 1, 2009; 587(7): 1439 - 1459.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
A. M. Hagenston, J. S. Fitzpatrick, and M. F. Yeckel
MGluR-Mediated Calcium Waves that Invade the Soma Regulate Firing in Layer V Medial Prefrontal Cortical Pyramidal Neurons
Cereb Cortex, February 1, 2008; 18(2): 407 - 423.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. D. Amaral and L. Pozzo-Miller
BDNF Induces Calcium Elevations Associated With IBDNF, a Nonselective Cationic Current Mediated by TRPC Channels
J Neurophysiol, October 1, 2007; 98(4): 2476 - 2482.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Hong and W. N. Ross
Priming of intracellular calcium stores in rat CA1 pyramidal neurons
J. Physiol., October 1, 2007; 584(1): 75 - 87.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. M. Power and P. Sah
Distribution of IP3-mediated calcium responses and their role in nuclear signalling in rat basolateral amygdala neurons
J. Physiol., May 1, 2007; 580(3): 835 - 857.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. Watanabe, M. Hong, N. Lasser-Ross, and W. N. Ross
Modulation of calcium wave propagation in the dendrites and to the soma of rat hippocampal pyramidal neurons
J. Physiol., September 1, 2006; 575(2): 455 - 468.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. N. Jacob, C.-U. Choe, P. Uhlen, B. DeGray, M. F. Yeckel, and B. E. Ehrlich
Signaling Microdomains Regulate Inositol 1,4,5-Trisphosphate-Mediated Intracellular Calcium Transients in Cultured Neurons
J. Neurosci., March 16, 2005; 25(11): 2853 - 2864.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. M. Power and P. Sah
Intracellular calcium store filling by an L-type calcium current in the basolateral amygdala at subthreshold membrane potentials
J. Physiol., January 15, 2005; 562(2): 439 - 453.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. Verkhratsky
Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons
Physiol Rev, January 1, 2005; 85(1): 201 - 279.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
I. Ismailov, D. Kalikulov, T. Inoue, and M. J. Friedlander
The Kinetic Profile of Intracellular Calcium Predicts Long-Term Potentiation and Long-Term Depression
J. Neurosci., November 3, 2004; 24(44): 9847 - 9861.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
H. Xing, A. Azimi-Zonooz, C. W. Shuttleworth, and J. A. Connor
Caffeine Releasable Stores of Ca2+ Show Depletion Prior to the Final Steps in Delayed CA1 Neuronal Death
J Neurophysiol, November 1, 2004; 92(5): 2960 - 2967.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. Kumar and T. C. Foster
Enhanced Long-Term Potentiation During Aging Is Masked by Processes Involving Intracellular Calcium Stores
J Neurophysiol, June 1, 2004; 91(6): 2437 - 2444.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. R. Young, S.-C. Chuang, and R. K. S. Wong
Modulation of afterpotentials and firing pattern in guinea pig CA3 neurones by group I metabotropic glutamate receptors
J. Physiol., January 15, 2004; 554(2): 371 - 385.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. Connor, L. D. Pozzo-Miller, and B. Ross
Synaptically driven Ca2+ release in hippocampal neurons
J Neurophysiol, November 1, 2003; 90(5): 3579 - 3579.
[Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
E. D. Martin and W. Buno
Caffeine-Mediated Presynaptic Long-Term Potentiation in Hippocampal CA1 Pyramidal Neurons
J Neurophysiol, June 1, 2003; 89(6): 3029 - 3038.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. W. Shuttleworth, A. M. Brennan, and J. A. Connor
NAD(P)H Fluorescence Imaging of Postsynaptic Neuronal Activation in Murine Hippocampal Slices
J. Neurosci., April 15, 2003; 23(8): 3196 - 3208.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
N. B. Pivovarova, L. D. Pozzo-Miller, J. Hongpaisan, and S. B. Andrews
Correlated Calcium Uptake and Release by Mitochondria and Endoplasmic Reticulum of CA3 Hippocampal Dendrites after Afferent Synaptic Stimulation
J. Neurosci., December 15, 2002; 22(24): 10653 - 10661.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. Zhou and W. N. Ross
Threshold Conditions for Synaptically Evoking Ca2+ Waves in Hippocampal Pyramidal Neurons
J Neurophysiol, April 1, 2002; 87(4): 1799 - 1804.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. Mannaioni, M. J. Marino, O. Valenti, S. F. Traynelis, and P. J. Conn
Metabotropic Glutamate Receptors 1 and 5 Differentially Regulate CA1 Pyramidal Cell Function
J. Neurosci., August 15, 2001; 21(16): 5925 - 5934.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. A. Reid, R. Fabian-Fine, and A. Fine
Postsynaptic Calcium Transients Evoked by Activation of Individual Hippocampal Mossy Fiber Synapses
J. Neurosci., April 1, 2001; 21(7): 2206 - 2214.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. G. Rae, D. J. Martin, G. L. Collingridge, and A. J. Irving
Role of Ca2+ Stores in Metabotropic L-Glutamate Receptor-Mediated Supralinear Ca2+ Signaling in Rat Hippocampal Neurons
J. Neurosci., December 1, 2000; 20(23): 8628 - 8636.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L. D Pozzo-Miller, J. A Connor, and S B. Andrews
Microheterogeneity of calcium signalling in dendrites
J. Physiol., May 15, 2000; 525(1): 53 - 61.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. A. Connor and R. J. Cormier
Cumulative Effects of Glutamate Microstimulation on Ca2+ Responses of CA1 Hippocampal Pyramidal Neurons in Slice
J Neurophysiol, January 1, 2000; 83(1): 90 - 98.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
L D. Partridge and C F. Valenzuela
Ca2+ store-dependent potentiation of Ca2+-activated non-selective cation channels in rat hippocampal neurones in vitro
J. Physiol., December 15, 1999; 521(3): 617 - 627.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Koizumi, P. Lipp, M. J. Berridge, and M. D. Bootman
Regulation of Ryanodine Receptor Opening by Lumenal Ca2+ Underlies Quantal Ca2+ Release in PC12 Cells
J. Biol. Chem., November 19, 1999; 274(47): 33327 - 33333.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
E. del Rio, J. A Bevilacqua, S. J Marsh, P. Halley, and M. P Caulfield
Muscarinic M1 receptors activate phosphoinositide turnover and Ca2+ mobilisation in rat sympathetic neurones, but this signalling pathway does not mediate M-current inhibition
J. Physiol., October 1, 1999; 520(1): 101 - 111.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
R. Bianchi, S. R. Young, and R. K. S. Wong
Group I mGluR Activation Causes Voltage-Dependent and -Independent Ca2+ Rises in Hippocampal Pyramidal Cells
J Neurophysiol, June 1, 1999; 81(6): 2903 - 2913.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
L. D. Pozzo-Miller, T. Inoue, and D. D. Murphy
Estradiol Increases Spine Density and NMDA-Dependent Ca2+ Transients in Spines of CA1 Pyramidal Neurons From Hippocampal Slices
J Neurophysiol, March 1, 1999; 81(3): 1404 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. Narasimhan, I. N. Pessah, and D. J. Linden
Inositol-1,4,5-Trisphosphate Receptor-Mediated Ca Mobilization Is Not Required for Cerebellar Long-Term Depression in Reduced Preparations
J Neurophysiol, December 1, 1998; 80(6): 2963 - 2974.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
V. P. Bindokas, C. C. Lee, W. F. Colmers, and R. J. Miller
Changes in Mitochondrial Function Resulting from Synaptic Activity in the Rat Hippocampal Slice
J. Neurosci., June 15, 1998; 18(12): 4570 - 4587.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
L. D. Pozzo-Miller, N. B. Pivovarova, R. D. Leapman, R. A. Buchanan, T. S. Reese, and S. B. Andrews
Activity-Dependent Calcium Sequestration in Dendrites of Hippocampal Neurons in Brain Slices
J. Neurosci., November 15, 1997; 17(22): 8729 - 8738.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online