Journal of Neurophysiology, Vol 68, Issue 5 1901-1905, Copyright © 1992 by APS

ARTICLES

Expression of NMDA channels on cerebellar Purkinje cells acutely dissociated from newborn rats

C. Rosenmund, P. Legendre and G. L. Westbrook
Vollum Institute, Oregon Health Sciences University, Portland 97201.

1. Conflicting evidence exists concerning the expression and properties of N-methyl-D-aspartate (NMDA) receptors on cerebellar Purkinje cells during development. We used whole-cell and single-channel recording to examine NMDA receptors on acutely dissociated Purkinje cells from newborn rats (postnatal day 0-4). 2. NMDA channels were present on > 80% of identified Purkinje cells and had pharmacological and single-channel properties that were indistinguishable from NMDA receptors on other neurons. In particular, responses were glycine-dependent and Mg2+ produced flickery open-channel block. 3. Our results demonstrate the transient expression of NMDA receptor/channels on Purkinje cells early in development. As NMDA receptors have been implicated in developmental plasticity in other regions of the CNS, a similar role is feasible during climbing fiber innervation of Purkinje cells.

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